Technical Research Assistant ll BWH Neurology
Brigham & Women's Hospital(BWH)

Works independently, under minimal supervision. Executes protocols involving a variety of routine and non-routine experiments at a professional level. Develops modification of existing techniques, as appropriate. In collaboration with other members of the research team, gives input to research design. Calculates, transcribes and graphs data.

Qualifications
PRINCIPAL DUTIES AND RESPONSIBILITIES:
1. Perform cell culture, primary neuron culture, iPSC culture maintenance, and in vitro assays, examine protein expression level e.g. by ELISA and western blot.
2. Perform genetic, biochemical and phenotype tests.
3. Execute general molecular biology.
4. Contribute to design of research protocols along with research associates; troubleshoots problems as they arise; initiates alternative protocols/approaches and modifies existing technique for better efficiency.
5. Maintains complete and accurate record of experimentation. Assists in the planning/development of daily and long-term research projects; assists in manuscript and meeting preparation.
6. Maintains resource inventories.
7. Performs all other duties/responsibilities as directed.
8. Coordinates daily schedule of experiments. Responsible for data management: collects, records, tabulates and presents data. Creates graphs of experimental, performs statistical tests, and discusses progress of work regularly with supervisor.

QUALIFICATIONS:
• Must have a Bachelor's degree in a related research setting. One year of research experience

• Must have experience in wet labs preferably with cell line/iPSC work.

SKILLS/ABILITIES/COMPETENCIES REQUIRED:
1. Strong research design, execution and analytical skills. Ability to work effectively and productively while being involved in several experimental protocols simultaneously. Knowledge of various laboratory techniques including molecular cloning, immunochemical assays and western blotting, proper sample handling and universal precautions. Knowledge of and ability to trouble shoot techniques such as plasmid construction, mammalian cell transfection, gel electrophoresis and sterile tissue culture.
2. Strong database statistical skills, written and verbal presentation skills. Attention to detail, accurate record-keeping skills, ability to evaluate and organize data for presentation, good oral and written communication skills.
3. Excellent time management/interpersonal/organizational skills. Work productively and effectively with focus under pressure. Able to complete assignments in a timely manner. Good interpersonal skills.

EEO Statement
BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.